Abstract
Objective(s):High Mobility Group Box 1 (HMGB1) is a nonhistone, DNA-binding protein that serves a crucial role in regulating gene transcription and is involved in various proinflammatory, extracellular activities. The aim of this study was to explore whether HMGB1 stimulation can upregulate the expression of PDL1.Materials and Methods:Thirty MS patients (n=30) with mild, moderate, and severe stages were recruited from the MS clinic. Blood samples were collected from patients, and monocyte cells were isolated from PBMCs using MACS brush separation. The isolated monocytes were cultured and stimulated with HMGB1 and HMGB2 over a time course of 0, 6, 12, 24, and 48 hours.
Results:The effects of HMGB1 and HMGB2 stimulation were evaluated over time. PDL1 expression increased significantly throughout the time course, with peak increases observed at 24 hours for HMGB1 and 12 hours for HMGB2 after stimulation.
Conclusion:Evidence from the present study suggests that enhanced PDL1 expression may result from HMGB1 and HMGB2 stimulation in MS patients.