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Biomedical Research Bulletin

Biomed Res Bull. 2024;2(4): 172-177.
doi: 10.34172/biomedrb.2024.23
  Abstract View: 11
  PDF Download: 9

Original Article

Glatiramer Acetate Effect on the Expression of MiR-21 and Let7i Via Down-Regulation of MAPK and JAK Signaling Pathways in Stroke Mice Model and PBMCs of Stroke Patients

Milad Golizadeh 1 ORCID logo, Nima Sadeghi 2, Paria Kahnamouyi 2, Reza Salehi 1* ORCID logo

1 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2 Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Author: Reza Salehi, Email: submission1999@gmail.com

Abstract

Background: Ischemic stroke is a prominent neurodegenerative disease, characterized by a decrease in the body’s functional abilities. It is proposed that many immunity members and cells might play a role in the pathogenesis of stroke, among which microRNAs have recently attracted much attention. MicroRNAs are noncoding RNAs involved in various immune functions and disorders. The present study aimed to evaluate the effect of Glatiramer acetate on microRNAs in stroke through immune signaling pathways.

Methods: In this study, photothrombotic (PT) stroke was induced in adult C57BL/6J mice. After three days of recovery, the mice were treated with Glatiramer acetate on day three post-stroke. Simultaneously, the peripheral blood mononuclear cells (PBMCs) were isolated from whole blood samples of the stroke patients. The expression of miR-21 and Let7i was evaluated using real-time polymerase chain reaction (PCR). Additionally, the expression of MAPK and JAK pathways was assessed by Western blot. The P value of 0.001 was considered statistically significant.

Results: Glatiramer acetate decreases the expression of let-7i and miR-21 in both the stroke mice model and PBMCs of stroke patients, compared to controls. Furthermore, Western blot analysis showed that Glatiramer acetate inhibits the immune signaling pathways involving MAPK and JAK in the stroke mice model.

Conclusion: In addition to its known neuroprotective effects during the acute phase of experimental stroke, Glatiramer acetate administration decreases the expression of miR-21 and let-7i via down-regulation of immune signaling pathways.


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Submitted: 12 Nov 2024
Revision: 29 Nov 2024
Accepted: 20 Dec 2024
ePublished: 29 Dec 2024
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