Faraz Norouzi-Bonab
1 
, Kimia Zabihi
1 , Mahsa Hasanzadeh-Moghadam
2, Seyed Zanyar Athari
3,4* 1 Faculty of Pharmacy, Eastern Meditation University, Famagusta, TRNC via Mersin 10, Turkey
2 Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3 Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4 Department of Medical Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract
Rapid brain extraction is essential for studies involving neural functions, such as electrophysiology and neurochemical experiments. Streamlining the pre-processing pipeline through rapid rat brain extraction improves research by avoiding bias and enhancing efficiency. The manual extraction of brains from rodents is time-consuming, which can affect research outcomes. Achieving fresh brain tissue is essential for experimental procedures in neuroscience, neurophysiology, and neuropharmacology, which are important for evaluating molecular alterations in proteins, mRNAs, and electrophysiology. This study focuses on introducing a rapid and simple technique for extracting a rat’s brain using a rodent skull awl-dilator. It also includes a video demonstrating both the traditional and novel approaches to brain extraction.