Maad Rayan Publishing Company Biomedical Research Bulletin 2980-9924 1 2 2023 06 29 Down-regulation of Toll-like Receptor 2 Via HMGB1 Blocking With Kaempferol on Kupffer Cells From Hepatocellular Carcinoma Patients 53 59 10.34172/biomedrb.2023.11 EN Zhang Yue https://orcid.org/0000-0001-8604-049X Tian Ma Xu Zhang Tong Ma https://orcid.org/0000-0001-5566-6772 Journal Article 10.34172/biomedrb.2023.11 2023 04 24 2023 06 19 Background: Kaempferol is a pharmacological kaempferol that can inhibit many inflammatory factors implicated in cancer. Methods: The present study focused on kaempferol function on the immunity signaling pathway. In this study, Kupffer cells were also isolated with new methods. Results: Our results indicated that the levels of interleukin 17 (IL-17), IL-33, and IL-6 in supernatants from cultured Kupffer cells from patients were increased after stimulation with high mobility group box-1 (HMGB1), but the levels of these cytokines decreased after using kaempferol to prevent inflammation. Conclusion: The findings of this study imply that the reduction of the Toll-like receptor 2 (TLR2) pathway and T helper 17 cell polarization may be due to HMGB1 blocking with Kaempferol in hepatocellular carcinoma.