Application and Immunogenicity of Aptamers in Cancer Therapy

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Introduction
The word aptamer comes from the Latin word "Aptus" which means inclusion.It is a single-stranded nucleic acid (DNA or RNA) or oligopeptide, specifically capable of binding to a high-affinity target. 1 Aptamers have many targets, ranging from small molecules and macromolecules to cells (prokaryotic and eukaryotic). 2Certain threedimensional (3D) spatial forms, such as stem-loops, hairpins, quadruplexes, triplexes, triple bonds, and the like, have been developed based on aptamers' nucleic acid arrangements.Aptamers have a high affinity for folding target molecules that can specifically bind to their destinations.Substances such as metal ions (Hg 2 + and Pb 2 + and the like), small organic molecules (amino acids, adenosine tri-amino acids, antibiotics, vitamins, and drugs), macromolecules (e.g., peptides, proteins, growth factors, dyes, toxins, pathogens, and biological markers), and microorganisms (e.g., bacteria) can be detected and measured due to the high affinity of aptamers for a variety of analytes.This article discusses a group of aptamers named oligonucleotides. 2Over the last decade, fluorescent semiconductor quantum dot compounds of aptamers have become an effective substratum for imaging and treatment of different illnesses (especially cancer) in animal and laboratory models. 3oreover, these conjugates have widespread potential to monitor the environment, diagnose diseases, and use bioassays.The study demonstrates recent discoveries of aptamer's immunogenicity properties in a variety of treatment applications.The single-stranded DNA, known as an aptamer, can bind to target molecules and disable them.Several researchers have also developed a more efficient DNA system to diagnose and treat infectious and cancer diseases. 4Scientists have improved the existing technologies for the synthesis of a modified DNA molecule called an aptamer.DNA aptamers are highly useful for pharmaceutical purposes since they can specifically bind to body molecular targets such as proteins, viruses, bacteria, and cells. 5DNA aptamers bind to the target and prevent it from functioning since they are manufactured synthetically.DNA aptamers have become an excellent technology for the diagnosis and delivery of medicines.However, no DNA aptamer has yet been authorized for clinical use because it is neither bound well nor digested easily by enzymes.Research by Yang et al revealed that a synthesizer is a DNA aptamer that can connect strongly to target molecules and has long-lasting stability to overcome these challenges.The results are published in the Scientific Reports journal. 6o solve the problem of poor binding, the research team of Yang et al added an abnormal base to the DNA aptamer (which has four main bases).The increased fifth component significantly enhances the aptamer's ability to attach to the target molecule (binding power increases 100-fold compared to conventional aptamer).Moreover, a small, unique part of DNA (called a mini-DNA clamp) is added to the DNA aptamer to avoid the simple digestion of the aptamer by enzymes. 7If the technology is commercialized effectively, DNA aptamers could also replace or supplement disease treatment antibodies.Antibodies such as aptamers are linked to specific targets but often lead to adverse immune reactions, whereas the extensive production process with high quality is not easy. 8Aptamers have unique properties, including being considerably smaller than antibodies and enzymes, making them superior to other detection molecules.The non-specific uptake of nucleic acids is usually weaker compared to proteins. 9DNA aptamers are commonly used for the design of a wide variety of pharmaceutical vaccines and medicines with high stability because of the aptamers' excellent immunogenicity.The main limitation of RNA aptamers is ribonuclease degradation, which is inhibited by diethylpyrocarbonate.The aptamer affinity of its particular analyte is occasionally up to ten thousand times greater than that of a similar molecule.This process is highly similar to a binding process for antigens and antibodies; thus, aptamers are sometimes called nucleotide antibodies. 10Therefore, aptamers' immunogenicity is extremely important today, especially in the vaccine industry and particularly in pharmacy.This study aims to evaluate the immunogenicity of aptamers in pharmaceutical sciences. 11

Aptamer Structure
Aptamer oligonucleotides are single-stranded or short-stranded RNAs that consist of approximately 20-80 nucleotides (5-15 kDa).Aptamers (Figure 1) can be found in complex folding structures such as quadruplex, pseudoknot, bulge, stem-loop, and aptamer oligonucleotides.These complex structures allow aptamers to bind specifically to their targets; in fact, aptamers identify their goals by structural recognition. 12

General Applications of Aptamers
Aptamers have a variety of applications, but their common ones include the measurement of substances or the diagnosis and treatment of diseases.They have unique characteristics that make them better than other detector molecules, such as anticorps.Aptamers seem to be extremely smaller than enzymes and anticorps.In comparison with proteins, the phenomenon of nonspecific absorption happens less often at nuclear acid levels. 13DNA aptamers are appropriate for the design of reusable and highly stable opto-sensors, whereas RNA aptamers provide a unit of measurement, and their main limitation is ribonuclease degradation, which is inhibited using diethyl pyrocarbonate-treated water. 14

Immunogenicity Properties of Aptamers Higher Stability Compared to Other Molecules
High temperatures cause proteins to lose their 3D structure, while oligonucleotides are thermally more stable.Aptamers change their structure without being degraded at temperatures above 60 °C and return to their original 3D structure when the temperature returns to room temperature, making aptamers a viable option for drug and vaccine immunization. 15The oligonucleotide aptamers were the most advantageous of all, and pharmaceutical researchers were persuaded to use aptamers as immunogens, in particular in the development of vaccines and other functional drugs.Aptamers have a denaturation-regeneration cycle and structure reversibility against various changes in physical conditions such as stress, temperature, pH, metal ions, and the like.While antibodies are unavailable in prepared forms, their denaturation is irreversible, and they are doomed to perish.Aptamers do not need to be stored at a low temperature and have a long shelf life. 17All these properties make aptamers good drugs since they can keep their immunogenic effects, such as activating defensive pathways, even in harsh environments that make other therapeutic molecules lose their functions. 18Figure shows the Aptamer technology and its application at a glance.

Production of Aptamers
Monoclonal antibody detection and manufacturing is a time-consuming and expensive process that includes screening vast numbers of colonies.Furthermore, largescale mammalian cell culture products are required for the financial success of antibodies.However, aptamers' chemical synthesization is easy, cheap, and in vitro.While easily maintained, transported, and synthesized, aptamers, once chosen, have a high analytic affinity and specificity with low dissociation and reproducibility syntheses; in addition, their chemical process is highly precise.Further, the ability of aptamers to be easily altered by various chemical reactions enhances their stability and nuclease resistance.Because of these properties, aptamers have proven to be highly effective in the development of vaccines and immunization drugs.Another immunogenic feature of aptamers is their easy chemical manipulation, allowing for a broad spectrum of modifications and the addition of a wide range of functional and chemical groups.Moreover, it is effortless to regenerate and reproduce modified surfaces using aptamers. 21

Synthesis of Aptamer Oligonucleotides for Immunogenicity
These aptamers are synthesized in the systematic evolution of ligands by the exponential enrichment (SELEX) cycle.This cycle is a practical approach to selecting highquality aptamers from a library, including many random oligonucleotides, for both vaccine development and immunization.A library with 10 13 -10 15 oligonucleotides is chemically generated in this cycle.These fragments each have an intermediate, random segment of approximately 60 nucleotides, which are utilized to select aptamers for medication and vaccine manufacture.They are selected with the best and most effective high immunogenic aptamer technique. 13Next, the library is used on a target (e.g., pure protein or cancer cells).In this way, aptamers with immunogenicity and unique properties get attached to the target. 22The connected parts are separated from the unconnected parts and then copied and given new targets as a new library.This cycle is repeated several times until the final selection, extension, and sequence are made of the most specific components with high immunogenicity.Over the years, this technique has been modified and improved at various stages to reduce the number of cycles and its duration in its new types 23 .The procedure is illustrated in Figure 3.

Immunogenic Application of Aptamers
Due to their oligonucleotide properties, such as their high specificity and purpose-related affinity, low cost of production, and easy binding to reporter molecules, they can replace antibodies in numerous fields, particularly in the pharmaceutical and vaccine industries.These programs involve identifying and processing diseases, biosensors, and drug carriers. 23For instance, in a program, two aptamers were developed that had anti-CD28 effects.CD28 is one of the main costimulatory receptors responsible for the proper activation of T lymphocytes.The program proved that these aptamers can be easily engineered, depending on the needs of the immunological approach, to function as either antagonistic (blocking) or agonistic (activating) by means of a simple dimerization. 24inally, it showed that agonistic anti-CD28 aptamers boost the antitumor immune response elicited by idiotypic vaccination in a murine lymphoma model, increasing the overall survival to a considerable extent. 25

Aptamers in Pharmaceutical Biotechnology and Pharmacy
The SELEX technique is used to select aptamers from a huge number of oligonucleotide sequences.Aptamers have several benefits over antibodies, which are provided as follows: Unlike antibodies, aptamers are produced in vitro using chemical procedures.Unlike antibodies, they detect a broad spectrum of target molecules, such as proteins, peptides, toxins, amino acids, drugs, metal ions, and even complete cells. 26In addition, chemical modification in aptamers is easier to do compared to antibodies.Further, unlike antibodies, aptamers' denaturation, which happens due to an increase in temperature, is reversible.Furthermore, aptamers can be more densely immobilized at the bioreceptor level in biosensors, which increases their sensitivity in identifying and measuring the target molecule.Moreover, many aptamers change conformations by binding to the target molecule, a feature that aids in the signal generation process in some biosensor systems.Aptamer applications are depicted in Figure 4.
Two types of target molecules are recognized by aptamers, including small and large (e.g., proteins) molecules.Aptamer is folded into a 3D structure in such a way that slits are created on its surface for the connection of small target molecules.As regards large molecules, the aptamer is folded in such a way that it takes place in the gaps and surface grooves of the large molecule. 27In the field of biotechnology, considerable attention is dedicated to aptamers because of the benefits of antibodies.These molecules may be employed as drugs to design biosensors and supply them to inhibit a particular protein, enzyme, or the like. 28

Acid-nucleic Aptamers: New Immunogenic Tools in the Process of Diagnosis and Treatment
Aptamers bind to specific targets, including proteins, peptides, and carbohydrates.Aptamers recognize targets and bind to them with a 3D structure, while antibodies recognize the linear epitopes of the targets.Considering that they are chemically synthesized, the process can simply be scaled up, not to mention that the batch-tobatch variation of the process is low, and the product is highly stable. 29Additionally, the production of aptamers is fast and cost-effective.Aptamers are highly safe biomaterials with no reported in vivo immunogenicity.This review article addresses the production method of nucleic acid aptamers, their advantages and disadvantages in comparison to antibodies, and their prospective applications in medicine (Figure 5).Aptamers' limitations and strategies to overcome them also underwent investigation. 30solation and Selection of High-Affinity Aptamer DNA Against Vascular Endothelial Growth Factor (Angiogenic Factor) for High Immunogenicity VEGF is a significant growth factor in the cell division and differentiation of tissue, which is needed for the synthesis and development of new blood vessels that are meant for the tissue transportation of oxygen and nutrients. 31s a result, this growth factor can be employed to stimulate angiogenesis in tissues using in vivo methods to treat damaged body tissues and in vitro methods for tissue engineering in medicine.On the other hand, in some situations, the body produces this component in an excessive and unregulated manner, which occurs  in disorders and diseases such as age-related macular degeneration (AMD), diabetic macular edema, and the appearance of malignant tumors. 32VEGF contains several different isoforms within the body.VEGF165 is the most abundant and noticeable isoform in the body, which plays a vital role in the process of tissue angiogenesis. 33EGF165 is also a helpful biomarker for cancer tumor diagnostics due to its increased cancer cell secretion.There is, therefore, a considerable necessity for cleansing and isolating this protein factor to use as a growth factor necessary for angiogenesis in the tissues and inhibiting its secretion or production to treat disorders caused by its unregulated secretion.Antibodies are one of the most important ligands utilized for protein isolation in chromatography while being used as medications. 34owever, there are some limitations to using antibodies, including immunogenicity, high biodegradation and instability, and high production costs.Today, novel nucleic acid ligands known as aptamers are utilized to solve this issue.The aptamer is a short single-stranded oligonucleotide sequence of RNA or DNA with a specific 3D form and the ability to attach specifically to the target protein with high affinity.In contrast to common anticorps, aptamers are not developed in biological systems but may be produced easily in the laboratory. 35he perfect example of the mentioned aptamers would be Pegaptanib, which exerts several physiological and pathological functions, such as an increase in vascular permeability causing fluid leakage in wet AMD. 36egaptanib selectively interacts with the heparin-binding site of the extracellular VEGF165 isoform and prevents VEGF165 from stimulating its receptor on the surface of the endothelial cell, thus blocking the initiation of the intracellular cascade and consequently inhibiting vascular permeability and retina neovascularization. 37

Selection of Aptamers by Systematic Evolution of Ligands by Exponential Enrichment Technology for Increased Drug Immunogenicity
The first step in the design of opt censors is the identification of a specific aptamer for the target molecule by an adaptive selection process called SELEX. 38In the 1980s, human immunodeficiency investigations were performed on the concept of protein binding to DNA.Studies have revealed that such viruses encode a variety of short RNAs that bind to cellular or viral proteins with high specificity and affinity.The SELEX process at the Goldo Zhostak Laboratory was reported in subsequent investigations in 1990.Aptamers are selected randomly from a synthesized oligonucleotide library during the SELEX procedure.SELEX is a hybrid method of chemistry that selects a single string from a library of 10 15 to 10 16 oligonucleotides with a length of 40 to 100 bp. 39Each strand is comprised of two fixed segments for amplification by polymerase chain reaction (PCR) on both sides of the oligonucleotides and one segment between them in a random variable sequence.In each SELEX procedure, a random DNA oligonucleotide library is developed, which is used directly for selecting DNA aptamers. 40his library must, first, be transformed into an RNA library to choose RNA aptamers.SELEX is defined by the repetition of five successive steps, including connection, separation, rinse, amplification, and conditioning.The library and target molecule will be incubated to connect in the first round of the SELEX procedure.Unbound oligonucleotides are removed by multiple vigorous wash steps from the connected complex. 41The oligonucleotides are washed before being amplified by RT-PCR or PCR.In this situation, a new enriched reservoir of selected oligonucleotides is produced by the PCR transcription of the suitable ssDNA from the SELEX results. 42This selected oligonucleotide resource is then used in the subsequent round of selection.To select specific aptamers for high-affinity target molecules, 6-20 rounds of the SELEX procedure are often necessary. 43Figure 6 sums up the SELEX procedure.
Despite the tremendous growth in the usage of aptamers for decomposition work as molecular detection elements, their interaction with target molecules necessitates the employment of a digital decomposition system.In reality, for the optimal usage of aptamer as a parser probe, it is crucial to establish a quantifiable signal from aptamertarget molecule interaction by simple approaches 44 .To date, multiple methods have been developed for measuring distinct molecules' properties, such as light transmission, mass-sensitive mass, or electrochemical measurements. 45any biosensors, however, are generated using electrochemical techniques.A biosensor is an analytical instrument in electrochemistry that transforms biological responses into electric output. 46

DNA Aptamer Immunogenicity
Many 3D compounds are formed by nucleic acid sequences such as DNA and RNA.Some of these structural variants can attach to proteins and tiny molecules.Oligonucleotides with adequate binding properties can be screened using a practical selection approach. 47Many pieces of research on the potential laboratory applications of aptamers have been published lately.According to these findings, aptamers play a crucial role in immunogenicity.Aptamers may be viable alternatives to immunological reagents and immuno-oligonucleotide compounds in some methods. 48

Effect of Aptamer on Immunoglobulin (as an Immunogenic Effect)
The interaction between aptamer RNA and human immunoglobulin G (IgG) has been identified by Japanese researchers.Aptamer Apt8-2 is a molecule that is 23 nucleotides long.This aptamer also contains two fluoropyrimidines that bind to the FC segment in human IgG with considerable affinity. 49However, this aptamer is unable to bind to the animal IgG.Apt8-2 competes with a protein for binding to FC in IgG.Apt8-2 competes with protein A, but not with the Fcγ receptor, for IgG binding.Nuclear magnetic resonance chemical-shift analyses localize the aptamer-binding sites on the Fc subdomain, which partially overlaps the protein A binding site rather than the Fcγ receptor binding site.The tertiary structures of the predicted recognition sites on the Fc domain differ significantly between human IgG and other species of IgGs; this, in part, accounts for the high specificity of the selected aptamer.Apt8-2 can, therefore, be used as a protein A alternative for the affinity purification of human IgG and therapeutic antibodies.Apt8-2 interaction with FC is detected only in the presence of Mg + 2 and Ca + 2 (The Apt8-2 response to FC is only noticed when Mg + 2 and Ca + are present).The aptamer bound to the IgG is released by removing these ions with ethylenediaminetetraacetic acid (EDTA).Acid buffers are also utilized to release IgG from protein A. As a result, acidic conditions can be deeply destructive to IgG.For this reason, it is recommended that Apt8-2 be utilized to release IgG because EDTA is used to extract IgG from Apt8-2. 50

Aptamer Reaction With Receptor Cells in the Immunogenic Process
Aptamer was previously employed to modulate a wide range of cellular activities in immune responses.Aptamer has the potential to block various cellular receptors and prevent their interactions with ligands.Various investigations have also revealed that aptamers can behave as stimulators of isolated reactions.Xiang et al combined different aptamers to minimize immunological reactions. 52In other trials, they could use aptamers that bind to proteins such as CD-28, which is involved in lymphocyte T activation.Many aptamers are capable of blocking the B7 connection to CD 28.Some anti-CD 28 aptamers can also function as agonists, triggering the anti-tumor cellular immune response. 53In addition, the anti-CD28 aptamers, which have been designed, show two different functions depending on which is needed, one being antagonistic (blocking) or agonistic (activating), which is related to dimerization.It has been demonstrated that the agonistic function can increase the immunogenicity of vaccination in a murine lymphoma model, resulting in higher survival rates. 54

Combination of Aptamer With Soluble Proteins
C5 is a part of the innate immune system that aids in the response against infectious pathogens.C5 proteolytic components are found in highly active inflammatory proteins.In C5, the proteolytic components are a portion of the inflammatory proteins that are highly active. 55C5a is a major cause of systemic inflammation in a variety of diseases, including sepsis and autoimmune disorders.Multiple types of aptamers have been synthesized to restrain C5 and its proteolytic components in this regard.The C5 protein binds various RNA molecules with dissociation constants comparable to those of M1 RNA, the RNA component of RNase P, one of the major ones being the W2 sequence.The W2 sequence has independent mg2+ interactions with C5, though the affinity between them increases with an elevated level of NH 4 + in the environment, suggesting the existence of hydrophobic bonds between them. 56The core RNA motif essential for interaction with the C5 protein was identified as a stem-loop structure, comprising a 5 bp stem and a 20 nt loop.Such aptamers as W2 can protect cells from proteolytic destruction by binding to C5 components.Nuclease-resistant aptamers (RNA/DNA NOX-D20) are synthesized from aberrant oligonucleotides.In humans and mice, RNA/DNA NOX-D20 can suppress C5 activity. 57

Immunogenic and Therapeutic Applications of Aptamers Application of Aptamer in the Field of Treatment
This section discusses aptamers that have entered the realm of treatment and are in various stages of clinical trials (Table 1).The first of these aptamers is Macugen, which has been invented by Pfizer and Eyetech and is currently commercially available for the treatment of AMD 58 .It is an aptamer conjugated with polyethylene glycol, a single-stranded nucleic acid specifically for VEGF-165, which plays a key role in angiogenesis and permeability. 59

REG1
It was invented as a novel aptameric anticoagulant drug.This drug (Figure 7) is an aptamer in phase two of clinical trials.It is made up of RB006 (coagulation factor 9-specific aptamer) and RB007 (aptamer RB006 oligonucleotide antidote). 60RB006 is a 2-ribopurine/2-fluoropyrimidine aptamer conjugated to 40 kDa polyethylene glycol to protect it from nuclease degradation. 61

AS1411
A detailed representation is displayed in Figure 8.It is a nucleolin-specific aptamer that is used to treat acute myeloid leukemia. 62ARC1779 ARC1779 (Figure 9) is a proprietary aptamer of the von Willebrand factor.This aptamer is prescribed to patients suffering from carotid artery disease.

NU172
It is a particular aptamer of thrombin, applied in various clinical stages as an anticoagulant (Figure 10). 63

Diagnostic Programs of Aptamers for Drug Safety
Aptamers can be applied to biomass through chemical modifications, either with luminescence or by binding to different nanoparticles (NPs).In one study, for example, DNA aptamer against Bacillus anthracis spores was produced in the form of a sandwich (an electrochemiluminescence magnetic sphere aptamer) to detect anthrax spores. 64,65Another diagnostic application is the identification of cancer cell biomarkers through the production of aptamers adverse to these markers, which are used to detect and image malignant tissue. 59

Conclusion
RNA self-assembling of diverse therapeutic oligonucleotide NPs has so far achieved tremendous progress in nanotechnology.These NPs are generally composed of RNA molecules (aptamers) and have great potential for therapeutic applications because of their features.Some of these characteristics are appropriate size, multiple capacity, minimal stimulation of the immune response of molecular production, intrinsic RNA, chemical nature of NPs through chemical synthesis with infinite accuracy and reproducibility, high-grade RNA purification, rapid return of drug activity, and strength.In this paper, some methods were presented for the production and selection of aptamers, along with a list of the advantages and disadvantages of these agents and their immunogenicity. 52Aptamers have advantages over antibodies that have created a new perspective for their use in diagnostic and therapeutic methods.Antibodies produced against the target molecule bind to the target protein only under physiological conditions (a narrow range of temperature, acidity, and ionic strength), whereas aptamers are not restricted in this way. 53ptamers have advantages over antibodies that have created a new perspective for their use in diagnostic and therapeutic methods.In principle, aptamer-based NPs and their varied immunogenicity will have different applications, and it is reasonable to conclude that nanotechnology will play a crucial role in expediting translation and development in the years ahead.

Figure 2 .
Figure 2. Aptamer Technology and its Application

Figure 4 .
Figure 4. Application of Aptamers in Pharmaceutical Biotechnology and Pharmacy

Figure 6 .
Figure 6.Selection of Aptamers With High Safety and Special Properties by SELEX Technology.Note.SELEX: Systematic evolution of ligands by exponential enrichment